WebRight , the number of dye-coupled astrocytes was measured in confocal images of biocytin-filled, streptavidin-AF488-labeled cells, and was similar during the L/D-phases. Article Snippet: 3D z-stacks of biocytin filled CA1 pyramidal neurons were acquired using a Zeiss LSM710 confocal microscope and saved as czi files. WebAug 31, 2016 · Office of Communication, Outreach and Development. Center for Biologics Evaluation and Research. Food and Drug Administration. 10903 New Hampshire Ave …
Immunostaining of Biocytin-filled and Processed Sections …
WebBiocytin-filled GABAergic cells expressed GFP or EYFP under the Pvalb, Sst, and Npy promoters, respectively, and its fluorescent protein content was enhanced post hoc with anti-GFP immunostaining (green, middle; A1–C1). In the principal neuron no GFP/EYFP signal (green, middle) was detected (D1). Scale bar, 10 μm. WebAug 7, 2009 · Simultaneous responses are shown from both locations, with increasing stimulation intensity near the blue electrode (B) and the red electrode (C). (D) Reconstruction of a biocytin-filled L5 pyramidal neuron showing the experimental setup. Dendritic recording pipettes are shown near the third bifurcation (875 μm from the soma, … chip free video to dvd converter
Structure and function of a neocortical synapse Nature
WebAug 1, 2011 · HEGCs and normotopic granule cells were filled with biocytin during whole-cell patch clamp recording in hippocampal slices from pilocarpine-treated rats. The apical dendrite of 86% of the biocytin-labeled HEGCs extended to the outer edge of the dentate molecular layer. The total length and branching of HEGC apical dendrites that penetrated … WebSummaryAutomatic TranslationDecember 31st, 2016. This protocol presents a method for the morphological recovery of neurons patched during electrophysiological recordings … WebNov 16, 2011 · Biocytin-filled cells were incubated with peroxidase-avidin-biotin complex (ABC, Vector Laboratories) and visualized with DAB-nickel sulfate. Subsequently, slices were mounted onto microscope slides and coverslipped with Mowiol. The biocytin-filled neuron reconstruction was performed with the aid of a Neurolucida 3D reconstruction … chip freezer