WebHuman Antibody Capture Kit, type 2 is designed for use with Biacore 8 series as the kit contains larger product volumes than Human Antibody Capture Kit. Anti-Human IgG (Fc) antibody is suitable for immobilization on sensor chip surfaces using the immobilization buffer included in the kit and Amine Coupling Kit. The regenera- WebHis Capture Kit, type 2 is intended for capture of histidine-tagged molecules as ligands in biomolecular interaction analyses using Biacore™ systems. The Anti-histidine antibody is suitable for immobilization on carboxyl derivatized surfaces using Amine Coupling Kit and the included Immobilization buffer.
His Capture Kit, type 2 - Cytiva
WebCapture Reagents Mouse antibody capture kit, type 2 Mouse antibody capture kit, type 2 Reagents for the capture of mouse antibodies, in component volumes larger than the standard mouse antibody capture kit. Sufficient for at least 16 immobilizations and 1600 regenerations. Order Product Overview Related Documents Order Table Product Support WebCytiva AMINE COUPL KIT TYPE 2 Fisher Scientific Home Products 501055337 Cytiva AMINE COUPL KIT TYPE 2 Manufacturer: Cytiva BR100633 Reagents for covalent … chistes de thor
Twin-Strep-tag® Capture Kit for SPR - Fisher Sci
WebNov 22, 2024 · Amine Coupling • efficient amine coupling depends on pre-concentration on the sensor and should be performed at a pH lower than the PI of the protein (0.5-1 pH unit below) but higher than 4 (the pKa of carboxy-methyl groups on the surface is ~3.6) • 10-50 µg/ml (~25 µg/ml) typical when preconcentration works Webcovalently immobilized after capture by amine coupling. Stabilization by amine coupling Preparation Condition Sensor Chip NTA by injecting regeneration solution (350 mM EDTA in running buffer or water, pH ~ 8.3) over both active and reference surface for 60 s. Include an Extra Wash command with running buffer to remove remaining traces of EDTA. WebHis Capture Kit, type 2 is designed for use with Biacore 8 series as the kit contains larger product volumes than His Capture Kit. The Anti-Histidine antibody is suitable for immobilization on sensor chip surfaces using the immobilization buffer included in the kit and Amine Coupling Kit. The regeneration chistes de tornillo