Web@journal { , title = {Fluorescent labeling of tetracysteine-tagged proteins in intact cells}, address = {}, booktitle = {Nature Protocols}, chapter = {}, edition ...
Fluorescein - Wikipedia
WebJun 28, 2024 · The fluorescein arsenical hairpin binder (FlAsH) fluorescein tag contains the amino acid sequence of CCPGCC, within which the tetracysteine motif is labeled with FlAsH-ETD2 fluorescein (Fig. 1b). Fig. 1. pOPHBL and pOPHFL bacterial expression vectors. (a) A map of the pOPHBL plasmid. DNA sequences and their amino acid coding … WebSep 3, 2024 · We used a panel of fluorescein arsenical hairpin (FlasH) bioluminescence resonance energy transfer (BRET)-based biosensors to monitor conformational changes in β-arrestin2 31,32. We also used two ... lightroom raw workflow
Characterization of Fluorescein Arsenical Hairpin (FlAsH) …
WebJan 20, 2024 · 3. fluorescein arsenical hairpin; FPR, formylpeptide receptor; GFP, green fluorescent protein; GIP, gastric inhibitory polypeptide; GLP, glucagon-like peptide; GPCR ... WebIntramolecular fluorescein arsenical hairpin (FlAsH) bioluminescence resonance energy transfer (BRET), in which both the fluorescence donor and acceptor are contained within the same protein, can be used to report on activation-induced changes in protein conformation. Websensors, the fluorescein arsenical hairpin binder (FlAsH)-binding motif CCPGCC was inserted at different positions within the in-tracellularloop-3(ICL-3)ofthereceptorintheCXCR4-CFPconstruct: between amino acids L226 and S227 (CXCR4-FlAsH226-CFP), be-tween amino acids H228 and S229 (CXCR4-FlAsH228-CFP), or peanuts saturated fat