WebApr 10, 2024 · In utero electroporation (IUE) is a technique that is widely used to introduce plasmids into NPCs in the brains of embryonic mice [16] [17] [18]. To perform IUE targeting the cerebral cortex, the ... WebIn utero electroporation, which was developed by combining electroporation with in utero surgery, has greatly facilitated functional analyses of genes through gain-of-function and loss-of-function approaches. DNA and RNA are efficiently transfected into the mouse embryo developing in the uterus in a spatiotemporally restricted manner.
Murine In Utero Electroporation Neuroscience JoVE
WebAug 20, 2014 · Since in-utero electroporation functions by transfecting mitotic precursor cells, we repeated our experiments in organotypic hippocampal slice cultures, where biolistic transfection occurs in post-mitotic neurons. The slice cultures were prepared from rat; however, the target sequence of CC_Grin1.1 is fully conserved between mouse and rat. WebApr 29, 2024 · Overexpression of this gene in embryonic mouse neocortex by in utero electroporation resulted in an increase in cycling BPs in the SVZ and intermediate zone, but not in cycling APs in the VZ . Moreover, this increase involved Tbr2 -expressing but not Sox2 -expressing BPs, indicating that bIPs rather than bRG were affected. hotels near daytona speedway
In Utero Electroporation in Mice SpringerLink
WebIn utero electroporation has been extensively used to study a variety of developmental questions in the developing brain. This protocol aims to provide the basic knowledge for a … WebFeb 4, 2016 · In utero electroporation was originally described in 2001, and it was further developed as a quick and simple method to genetically manipulate pyramidal neurons of … WebSep 1, 2016 · In utero electroporation is a laboratory technique widely used to introduce transgenes into tissues in developmental biology studies, especially in brain development (Fukuchi-Shimogori and Grove, 2001; Saito and Nakatsuji, 2001; Tabata and Nakajima, 2001).This technique has provided various useful toolkits that enable the modification of … hotels near dayton masonic center