Iptg x-gal spectinomycin table
WebMar 16, 2010 · Pspac = IPTG inducible promoter, PdivIVA = constitutive B. subtilis divIVA promoter used to drive expression of lacZ, AmpR = ampicillin resistance gene, SpcR = spectinomycin resistance gene. Plasmid pLK has been made available through the Bacillus Genetic Stock Centre, with accession number ECE219. WebOur X-Gal and IPTG products are intended to be used together for blue-white colony screening. X-Gal (5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactoside) is a chromogenic …
Iptg x-gal spectinomycin table
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WebTo screen bacterial colonies, the chromogenic substrate X-Gal and the gratuitous inducer IPTG are mixed with suitable dilution of a culture, combined with molten top agar, and … WebJun 6, 2024 · The growth temperature was 37°C. The antibiotic concentrations used are as follows: 75 µg/mL ampicillin, 50 µg/mL kanamycin, 25 µg/mL chloramphenicol, 50 µg/mL spectinomycin, and 15 µg/mL tetracycline. X-gal and isopropyl β-D-thiogalactopyranoside (IPTG) were used at 25 µg/mL and 0.5 mM, respectively.
Web6. 1 μL of X-Gal Solution (20 mg/mL), ready-to-use. 7. 1 μL of 100 mM IPTG Solution, ready-to-use. 8. Mix well. 9. Pour 25 mL of prepared LB agar into each Petri dish. 10. Dry opened LB plates at room temperature under UV light for about 30 minutes. This protocol is for the Preparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening. WebPreparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening For individual LB (Luria Broth) agar plates: 1. Pour sterile warm LB agar (about 25 mL) into a Petri dish. 2. …
WebFig. 2. Blue plaques resulting from different X-Gal and IPTG treatments of electro-transformed E. coli cells. All pictured samples received 10 pg M13mp19 DNA. A) Sample 3, Table 1: electro-transformation plated on an X-Gal and IPTG plate. B) Sample 6, Table 1: 50 µl 20 mg/ml X-Gal and 10 µl 20 mg/ml IPTG added to cells after electroporation.
Webchloramphenicol and spectinomycin were added at concentrations of 100 mg/ml, 10 mg/ml, 30 ... respectively. IPTG and X-gal for blue/white selection were added at concentrations of 0.1 mM and 40 µg/ml, respectively. 2.2 Constructing gRNA plasmid and donor DNA To construct gRNA plasmid, a set of primers was used to PCR amplify the pGRB backbone ...
WebChromoMax IPTG/X-Gal Catalog # 13988100 13988101 13988102 BP42001 BP420010 BP420050 SDS ChromoMax™ IPTG/X-Gal Catalog # 10133443 10225163 10658593 ACRBP4200 BP4200 BP42001 BP420010 BP420050 SDS 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL), Ultrapure, Thermo Scientific Catalog # ALFAAJ10077 SDS … ordered phase temWebDans les colonies produisant la β-galactosidase, l'X-gal est clivé avec d'un côté le galactose et de l'autre le noyau indole portant le chlore et le brome qui donne une couleur bleue aux dites colonies. l'allolactose (galactose-(β1→6)-glucose) est un isomère du lactose (galactose-(β1→4)-glucose) et l'inducteur de l'opéron lac. Le ... iremit ellice winnipegWebThe IPTG/X-GAL Solution is a ready-to-use product in an easy-to-use spray bottle format. The solution concentration is 8mg/ml. Each pump of the spray bottle produces about 0.2ml of solution. Compare this item LB Agar Plates with … ordered pitch class intervalsWebIPTG, X-gal, speciinomycin Based on your answers to the previous questions, fill in Table with your predictions of whether there will be bacterial growth on each plate: Okay, So 63 … ordered pitch intervalWebTransform the reaction product to XL1-Blue and streak the transformant on Ampicillin/X-gal/IPTG LB plate. Culture O/N at 37°C. Screen for correctly assembled clones by colony PCR. You can use TALE-F/TALE-R primers both for ptCMV-153/47-VR and ptCMV-136/63-VR vectors. The detailed reaction condition is described in Appendix. ordered pairs x yWebApr 4, 2024 · (D) The E. coli K-12 BW25113 WT and ∆ lon strains harboring the plasmid pCA24N-NHis-yefM were induced with 0.5 mM IPTG for 30 min, and 1% spectinomycin (100 µg/mL) was added to the strains to activate a stress response. We collected equivalent quantities of cells at 0 min, 30 min, 60 min and 120 min and then ran Tricine-SDS-PAGE … ordered pairs vs unordered pairsWebTable 2. Starter plate conditions. Starter Plate Bacterial Cas9 DNA Repair System SGRNA Donor Plate Additives Colony Color Template DNA IX IPTG, X-gal Blue OFF IX/ARA IPTG, X-gal, Blue ON arabinose A. Using evidence from Table 2, explain in complete sentences why the bacterial colonies on the starter plates are blue. B. ordered pizza say 2 wds crossword