WebPhosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. WebThe cells were labeled with ERK1 + ERK2 Antibody [ERK-7D8] (ab54230) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 ® Rabbit Anti-Mouse IgG Secondary Antibody at a dilution of 1/400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI.
ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total …
WebThe p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ... WebMar 10, 2024 · p-ERK Antibody (E-4) is recommended for detection of ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat, human and origin by … how long can a submarine be
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) …
Web製品の概要. Abcam’s ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA kit is designed for the semi-quantitative measurement of ERK1/2 (pT202/Y204) and Total ERK1/2 protein in Human and mouse cells. The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody ... WebWestern blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686) Peptide Competition: Extracts prepared from HEK293 cells transiently transfected with plasmids expressing … WebThe immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. how long can a stick of butter be left out