Phospho flow antibodies
WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. WebPolyclonal Antibody for studying PLCg1 (Tyr783) phosphate. Cited in 265 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously …
Phospho flow antibodies
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WebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody. WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: Akt1, Akt2, Akt3, PKBα, PKBβ, PKBγ, RAC-PKα, RAC-PKβ, RAC-PKγ, STK-2 Reactivity: Human (QC Testing), Mouse (Tested in Development) Isotype: Mouse BALB/c IgG1, κ Immunogen: Phosphorylated Human Akt1 (pS473) Peptide
WebJun 5, 2015 · The eight phospho-specific antibodies were chosen to represent major signaling nodes responsive to cytokine, TLR, and antigen receptor signaling. This antibody … WebApr 9, 2024 · “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation” The idea is that the antibody is: ‘pan’ in that it will react with all phospho-histidine residues on proteins, but ‘specific’ in that it will not react with unphosphorylated histidine residues or other phospho-aminoacids e.g. phospho-serine. Share
WebAll lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate Lane 2 : Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate Lane 3 : Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 µg. Then the membrane was incubated with … WebPhospho-S6 (Ser235, Ser236) Antibody (12-9007-42) in Flow. Intracellular staining of freshly-harvested (left), unstimulated (middle), or 30-minute LPS-stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human phospho-S6 (S235/S236) PE, using the Intracellular Fixation and ...
WebFlow cytometry offers a quick and effective way to measure signaling at a specific time point in individual cells. The staining methods for detecting signaling molecules and …
WebAnti-Phosphoserine antibody See all Phosphoserine primary antibodies Description Rabbit polyclonal to Phosphoserine Host species Rabbit Specificity Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. songs with good beatsWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free. Suitable for: ELISA, Dot blot, WB, IHC-P. Reacts with: Human. small glass corked bottlesWebPhospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb detects endogenous levels of Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. Species Reactivity: Human, Mouse, Rat Source / Purification songs with good figurative languageWebPE anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody - ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ... Each lot of this … small glass cylinder vases dollar treeWebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. songs with good bass linesWebPhosphoflow is a specialized application of flow cytometry that enables researchers to examine the phosphorylation status of specific proteins that govern cellular responses … songs with good drum beatsWebThis Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is … small glass curio cabinets display case