WebqTower3/G thermocyclers for use with the Assurance SARS-CoV-2 Panel, (3) addition of the Apto-Gen One-step and AzuraQuant 1-step Probe NoRox qPCR master mixes for use with the Assurance SARS-CoV-2 ... Weband a qTOWER3 G qPCR cycler (Analytik Jena, Jena, Germany). All assays were performed in a duplex real-time PCR format with a specic primer-probe mixture (Table 3) and using the porcine glyceraldehyde-3-phos-phate dehydrogenase (pGAPDH) gene as a reference. A reaction volume of 20 µl was prepared containing 1.8 µl

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